Comparing lethal dose of povidone-iodine and virkon® to the acanthamoeba cyst: In vitro study.
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Date
2008-07
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Abstract
Objective: To compare the least concentration of Povidone-iodine and Virkon®, phenolic-based compound with accelerated
hydrogen peroxide and potassium peroxymonosulfate, which can cause a lethal effect to the cyst form of the Acanthamoeba
spp.
Methods: Acanthamoeba spp. was isolated from the keratitis patient and was cultured using amonoxenic medium
supplement with heat killed Escherichia coli and incubated for up to seven days for the production of mature cysts.
Acanthamoeba cysts on the culture plates were mixed with several dilutions of Povidone-iodine to compare to several
dilutions of Virkon®. After incubation for 1 hour, they were washed and centrifuged to remove the chemical supernatant.
The pellets of the mature cyst were viewed by a light microscope for seven days, and further recultured on the monoxenic
medium to confirm that the cysts were all died (cysticidal) for seven days.
Results: Povidone-iodine 0.04% and Virkon® 0.25% solutions were the least concentrations which could cause a lethal
effect to the Acanthamoeba cyst.
Conclusion: Our results demonstrated that both the Povidone-iodine and Virkon® showed antiamoebic activity. The further
study should be done to determine whether Povidone-iodine and Virkon® can be used as a disinfecting solution for contact
len cases.
Description
Keywords
Acanthamoeba, Povidone-iodine, Virkon®
Citation
Roongruangchai Kosol, Roongruangchai Jantima, Kaewmanee Somkid, Manoot Daunphen. Comparing lethal dose of povidone-iodine and virkon® to the acanthamoeba cyst: In vitro study. Siriraj Medical Journal, 2008 Jul; 60(4): 187-189.