Antibody detection against HPV16 E7 & GP96 fragments as biomarkers in cervical cancer patients.
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Date
2009-11
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Abstract
Background & objectives: Cervical cancer is the second most frequent cancer among females worldwide,
especially human papilloma viruses (HPV) types 16 and 18. In viral systems the identification of
serological markers would facilitate the diagnosis of HPV infections and virus-related disease. The aim
of the present investigation was to determine and search for serologic markers in cervical cancer patients
associated with HPV.
Methods: A total of 58 Iranian women with invasive cervical carcinoma including adenocarcinoma and
squamous cell carcinoma (SCC) were included. Serum antibody response to HPV infections in patients
was detected by Western blot and ELISA techniques based on recombinant HPV16E7 and the N-terminal
and C-terminal fragments of gp96 (NT-gp96 and CT-gp96) proteins. These recombinant proteins were
expressed in Escherichia coli as a His-tag protein and purified using affinity chromatography.
Results: The ELISA results indicated that patients with high antibody response to HPV16E7 had
significant seroreactivity to CT-gp96 fragment. In Western blot analysis, a strong association between
anti-E7, anti-NT-gp96 and anti-CT-gp96 reactivity and cervical cancer was obtained using purified
recombinant proteins. In adenocarcinoma cases, no significant difference was observed in seroreactivities
between normal and patients.
Interpretation & conclusions: The evaluation of cervical cancer patients' seroreactivities against three
recombinant proteins (rE7, rNT-gp96 and rCT-gp96) showed significantly higher levels of these markers
in SCC only, but not in adenocarcinoma and control groups. Also, the usage of both techniques (ELISA and Western blotting) can provide more reliable tools for diagnosis of cervical cancer.
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Keywords
Cervical cancer, E7, GP96, HPV, serological marker
Citation
Bolhassani Azam, Zahedifard Farnaz, Taslimi Yasaman, Taghikhani Mohammad, Nahavandian Bijan, Rafati Sima. Antibody detection against HPV16 E7 & GP96 fragments as biomarkers in cervical cancer patients. Indian Journal of Medical Research. 2009 Nov; 130(5): 533-541.