Immunolocalization of CD 34 positive progenitor cells in healthy human gingiva - a pilot study.
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Date
2009-06
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Abstract
Background & objectives: The gingiva is a tissue with a high turnover rate of both epithelial and
connective tissue cells. In an attempt to identify the possible source of cells which maintain the tissue
turnover, we used CD 34, a well established marker of peripheral blood stem cell in healthy human
gingiva to determine the origin of progenitor cells in healthy gingiva.
Methods: Healthy human gingival samples (n=15) were collected from patients undergoing orthodontic
extraction. Immunohistochemistry was done on 5 micron paraffi n fi xed section using the primary
antibody CD34 and a universal secondary immunoperoxidase kit. The sections were examined for a
golden brown stain indicative of a positive staining.
Results: Of the 15 samples 12 demonstrated a positive staining for the endothelial cells. Of these 12
samples, 11 demonstrated positive staining for stromal and paravascular cells and 10 a positive staining
for the basal epithelium layers.
Interpretation & conclusions: The presence of CD 34 positive cells in gingiva in stromal, paravascular
location, and basal layer of the gingival epithelium was demonstrated. We speculate that these could
be fi broblastic progenitors originating from the peripheral blood stem cells and the positivity stained
epithelial cells could be gingival epithelial stem cells.
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Keywords
CD 34 expression, gingival, immunohistochemistry, progenitor cells
Citation
Lavu Vamsi, Padmavathy R, Rao Suresh R. Immunolocalization of CD 34 positive progenitor cells in healthy human gingiva - a pilot study. Indian Journal of Medical Research. 2009 June; 129(6): 685-689.