Estimation of Cochlodinium polykrikoides growth potential using a dialysis membrane.
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2008-11-21
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We developed a test to measure the growth potential of C. polykrikoides using a dialysis membrane and artificial seawater. Nitrite nitrogen and inorganic phosphorus in the medium were almost completely removed when the medium was dialyzed against artificial seawater for five or more 6-hour cycles using a dialysis membrane (Spectrum's Spectra/Por 7 Membrane) with a molecular-weight cut-off of 50,000, regardless of the presence of C. polykrikoides. The phytoplankton grew well even after dialysis. To estimate the growth potential of C. polykrikoides, a minimum initial concentration of > 100 cells/ml is required. Methods using short-term starvation culturing of C. polykrikoides to measure growth potential were determined to be ineffective; instead, controlled tests using artificial seawater are recommended. The dialysis membrane used in this study can also be employed to measure the algal growth potential of other phytoplankton species.
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Lee YS. Estimation of Cochlodinium polykrikoides growth potential using a dialysis membrane. Journal of Environmental Biology. 2008 Nov; 29(6): 827-30