Isolation of cadmium-induced DNA sequence in microalga Nannochloropsis oculata.
No Thumbnail Available
Date
2008-07-07
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The gene induced in response to Cd exposure in microalga Nannochloropsis oculata was examined in this investigation. In order to isolate this gene by using nested PCR, degenerated primers were designed on the basis of highly conserved regions of the amino acid sequences of various phytochelatin synthase. The size of the nested PCR amplification product from Nannochloropsis oculata by using two degenerated primers was measured to be 266 bp. The 266 bp DNA fragment was sequenced, and it might be induced by Cd exposure in microalga Nannochloropsis oculata.
Description
Keywords
Citation
Min JK, Rhim TY, Shin HW, Lee MY. Isolation of cadmium-induced DNA sequence in microalga Nannochloropsis oculata. Journal of Environmental Biology. 2008 Jul; 29(4): 457-60