Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility.

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Date
2001-12-25
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Abstract
The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
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Dincturk HB, Dunn AJ, Jones DH. Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility. Journal of Biosciences. 2001 Dec; 26(5): 635-40
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https://imsear.searo.who.int/handle/123456789/111330
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Journal of Biosciences