Tyrosine phosphorylation of the human guanylyl cyclase C receptor.
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2000-12-20
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Abstract
Tyrosine phosphorylation events are key components of several cellular signal transduction pathways. This study describes a novel method for identification of substrates for tyrosine kinases. Co-expression of the tyrosine kinase EphB1 with the intracellular domain of guanylyl cyclase C (GCC) in Escherichia coli cells resulted in tyrosine phosphorylation of GCC, indicating that GCC is a potential substrate for tyrosine kinases. Indeed, GCC expressed in mammalian cells is tyrosine phosphorylated, suggesting that tyrosine phosphorylation may play a role in regulation of GCC signalling. This is the first demonstration of tyrosine phosphorylation of any member of the family of membrane-associated guanylyl cyclases.
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Bhandari R, Mathew R, Vijayachandra K, Visweswariah S. Tyrosine phosphorylation of the human guanylyl cyclase C receptor. Journal of Biosciences. 2000 Dec; 25(4): 339-46