The amino-terminal domain of human signal transducers and activators of transcription 1: overexpression, purification and characterization.
No Thumbnail Available
Date
2005-12-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The dual functional signal transducers and activators of transcription (STAT) proteins are latent cytoplasmic transcription factors that play crucial roles in host defense. Animals that lack these proteins are highly susceptible to microbial and viral infections and chemically induced primary tumours. We have over expressed the amino-terminal domain of human STAT1 (hSTAT1) in Escherichia coli and purified it by affinity chromatography and gel filtration chromatography. The entire process has been monitored by gel electrophoresis. The pure protein has been characterized by mass spectrometry and 2-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Our results indicate that the N-terminus of hSTAT1 exists as a dimer in solution.
Description
Keywords
Citation
Prabhu A, Coutinho E, Srivastava S. The amino-terminal domain of human signal transducers and activators of transcription 1: overexpression, purification and characterization. Journal of Biosciences. 2005 Dec; 30(5): 611-8