Colorimetric determination of piperazine.
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Date
1976-07-01
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Abstract
A sensitive method for the quantitative determination of piperazine is described. The method is precise and responds linearly from 25 g to 500 mug and above of the material. The procedure is based on the formation of a complex of piperazine with reineckate in neutral or acid medium. The complex can be separated by centrifugation. It is then dissolved in acetone and estimated at 530 nm in a colorimeter. Piperazine present in trichloroacetic acid extractrs of biological samples can also be estimated by this method.
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Sasi PK, Raj RK. Colorimetric determination of piperazine. Indian Journal of Physiology and Pharmacology. 1976 Jul-Sep; 20(3): 180-2