Browsing by Author "Singh, A"
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Item 18S rDNA sequencing aided diagnosis of Acanthamoeba jacobsi keratitis -A case report(All India Ophthalmological Society, 2019-11) Singh, A; Acharya, M; Jose, N; Gandhi, A; Sharma, SIdentification of Acanthamoeba cysts and trophozoites in cases of keratitis is traditionally done with microbiological techniques such as smear examination with 10% potassium hydroxide (KOH) and culture. Double walled cyst with hexagonal inner wall is characteristic of Acanthamoeba. We report a unique case of a 9 year old boy who presented with dense anterior corneal stromal infltration, which on smear examination showed atypical double walled spherical cysts, leading to a diagnostic dilemma. An 18S rRNA gene-based PCR done on the growth on culture, subsequently identifed a rarely reported species of Acanthamoeba. The patient was advised combination therapy with polyhexamethylene biguanide (PHMB 0.02%) and chlorhexidine (0.02%) eye drops. Three weeks post treatment, the keratitis resolved with scarring and vascularisation and visual acuity improved to 20/60. At 8 weeks follow up Best corrected visual acuity further improved to 20/30 with contact lens.Item Accidental poisoning in children.(1996-01-01) Singh, A; Choudhary, S RItem Acute nephritis in Kashmiri children--a clinical and epidemiological profile (a study of 350 cases).(1979-11-01) Manhas, R S; Patwari, A; Raina, C; Singh, AItem Acute pulmonary edema as a complication of anti-snake venom therapy.(2001-01-10) Singh, A; Biswal, N; Nalini, P; Sethuraman,; Badhe, APolyvalent Anti-snake Venom (ASV) is a life-saving antivenin for severe envenomation due to snake bite in India. ASV infusion is occasionally associated with severe allergic reactions, i.e. anaphylaxis and death. We report a rare instance of non-cardiogenic pulmonary edema due to ASV infusion in an eleven years old boy.Item Addressing the health needs of people with disabilities in India(Indian Public Health Association, 2020-03) Senjam, SS; Singh, AIn India, the number of people with disabilities is continuously growing over the past few decades. The figure is expected to increase due to population aging, with a resultant increase in chronic health conditions. The health of people with disabilities is a relatively neglected and ignored area. Further, the disabled face poor access to healthcare and frequently encounter discrimination or stigmatization. These situations make them more vulnerable to many comorbidities in their health, making severe compromises in their quality of life. Therefore, people with disabilities need special healthcare than people without disabilities. There is a need for sensitization of all health-care providers to ensure quality, affordable, and accessible health-care services for people with disabilities. To address the health-care needs of people with disabilities to the maximum, Ministry of Health, Government of India should incorporate appropriate guidelines in various national health programs and work together with a relevant ministry.Item Alterations in the systemic acid-base status and blood gas dynamics during progressive hypocalcaemia in cow calves.(1996-04-01) Setia, M S; Singh, A; Kahlon, R S; Randhawa, S SHypocalcaemia, induced in cow calves by slow infusion of Na2EDTA for 6 hr, led to biphasic changes in acid-base status. Decrease in ionized plasma Ca2+ concentration upto 0.82 +/- 0.06 mmole 1(-1) was associated with metabolic alkalosis. Further decrease in ionized plasma Ca2+ concentration (0.61 +/- 0.09 mmole 1(-1)) led to metabolic acidosis, superimposed by a respiratory component. There was significant decrease in oxygen tension and saturation of arterial blood as well as impaired uptake of oxygen by peripheral tissues and pulmonary blood.Item Anticonvulsant and analgesic activity of 1,2,4-thiadiazoles--a preliminary report.(1987-04-01) Pandeya, S N; Singh, AItem Antidiabetic and antiulcer effects of extract of eugenia jambolana seed in mild diabetic Rats : Study on gastric mucosal offensive acid-pepsin secretion.(2009-04) Chaturvedi, Aditi; Bhawani, G; Agarwal, P K; Goel, Shalini; Singh, A; Goel, R KDiabetes has been reported to increase propensity to peptic ulceration through its effect both on offensive and defensive mucosal factors. Seeds of Eugenia jambolana (EJ) have been reported to have both antidiabetic as well as ulcer protective effects. The present study evaluates the antidiabetic effects of ethanolic extract of dried seed kernel of Eugenia jambolana (EJE) and its comparative effect on gastric ulceration and acidpepsin secretion with standard antisecretory FL-blocker. Ranitidine and antidiabetic glibenclamide with a premise that Eugenia jambolana may show better ulcer healing effects by promoting defensive or reducing offensive mucosal factors in mild diabetes (MD) rats. MD was produced in adult rats by administration of streptozotocin (45 mg/kg, ip). EJE was given orally in the doses of 100–400 mg/kg for 10 days and in the dose of 200 mg/kg for 30 days respectively to study its dose- and time-dependent effects on various diabetic parameters like blood glucose, serum cholesterol and triglycerides, insulin level and glycosylated hemoglobin. For ulcer protective and gastric secretion studies, EJE (200 mg/kg) was given orally for 10 days against 2 h cold restraint stress (CRS)-, 4 h pylorus ligation (PL), aspirin (ASP, 200 mg/kg, 4 h) – and 95% ethanol (EtOH, 1 ml/200 g, 1 h)-induced gastric ulcers and offensive acid-pepsin secretion after 4 h PL with cooccurring MD in rats. EJE showed dose-dependent decrease in blood glucose level in MD rats. Blood glucose level remained stable in mild diabetic rats from 3rd day onwards after streptozotocin administration (taken as 1st day for treatment) and EJE (200 mg/kg) showed anti-hyperglycemic effect on 10th day of its administration. Further, EJE in the above dose also decreased cholesterol level with little or no effect on triglycerides level and reversed the decrease and increase in insulin and glycosylated hemoglobin level near to the normal level as observed alter 30 days treatment in MD rats. MD rats exhibited an increased propensity to gastric ulceration induced by CRS, ASP, EtOH and PL and caused increase in acid-pepsin secretion. EJE was not only effective in reversing the increased propensity to ulceration in diabetic rats but also decreased the acid-pepsin output better than glibenclamide. The ulcer protective effect of Eugenia Jambolana seems to be due to its antidiabetic and gastric antisecretory effects.Item Antigenic relatedness between enterotoxins of Escherichia coli associated with adult and infantile diarrhoea.(1981-02-01) Tabaraie, B; Sehgal, S C; Singh, A; Vadehra, D VItem An appraisal of microbiological spectrum in Acne Vulgaris from a tertiary care teaching institution.(2015-07) Shamsi, M S; Goel, S; Singh, A; Gupta, A; Bhardwaj, A; Chhoker, V K; Surana, A; Singh, L KBackground: Acne vulgaris is one of the most common skin disorders in puberty. The widespread and indiscriminate use of antibiotics in the treatment of acne has resulted in the spread of resistant bacterial strains and treatment failure. Aim: The study aimed to analyze the microbiological spectrum in acne vulgaris and to evaluate its susceptibility to the antibiotics widely used for acne. Material and methods: The present cross sectional study was carried out by the Department of Microbiology and Dermatology, MSDS Medical College, Fatehgarh among the patients seeking care for acne vulgaris with pustular and nodulocystic skin lesions in the Dermatology OPD of a rural medical school from western Uttar Pradesh. Patients with pregnancy or endocrinal problems like hirsutism, menstrual dysfunction or adrenal dysfunction and those taking drugs or contraceptives were excluded. The samples were cultured individually on blood agar and Muller-Hinton media. The cultures were then incubated under both aerobic and anaerobic conditions for 2-7 days . Bacteria were identified and their resistance to common antibiotics was evaluated according to the standard procedures. After compilation of collected data, analysis was done using Statistical Package for Social Sciences, version 21 (IBM, Chicago, USA). Results: Aerobically, Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne and Micrococcus spp were detected in 44%, 47%, 1% and 46% of samples respectively whereas anaerobically these were 39%, 21%, 34% and 1% respectively. Propionibacterium acne Staphylococcus epidermidis and Staphylococcus aureus were more sensitive to rifampin (almost 90%) compared to other drugs. Neomycin was found to be least sensitive. Conclusion: The study highlighted the need to discourage antibiotics' abuse. Rifampin is a suitable antibiotic for acne patients, but to achieve a better result, combination of rifampin with other antibiotics should be tried. It is also suggested that studies with bigger sample size on evaluation acne vulgaris treated by rifampin should be undertaken.Item ARA diagnostic criteria for rheumatoid arthritis: an obituary.(1987-09-01) Chopra, A; Subramanian, A R; Raghunath, D; Singh, AItem Association of polycythaemia, polycystic kidneys, diabetes mellitus and portal hypertension.(1973-08-16) Singh, A; Singh, S; Singh, NItem Atypical germ cells in a cryptorchid testis--a case report.(1991-07-01) Gupta, M K; Gupta, A K; Sarwal, V; Singh, AItem The baneful alliance of COVID-19 and mucormycosis: Histopathological concerns(Wolters Kluwer - Medknow, 2023-03) Singh, A; Kumar, V; Kumar, V; Verma, N.Background: Mucormycosis, once a rare fungal disease, has now shown a surge in cases, and its epidemiology in India is intriguing. Rising incidence confers the necessity of defining the demography of the population at risk, early diagnosis, and recommendations for the management of mucormycosis in patients with coronavirus disease (COVID-19) infection. Aims: To study the clinico-epidemological profile, risk factors, and histopathological features of COVID-19-associated Mucormycosis (CAM). Setting and Design: This is a retrospective observational study. Material and Methods: Fifty-six biopsy specimens taken from patients of COVID-19 with clinical suspicion of mucormycosis were included. Results: Diabetes mellitus, corticosteroids, and oxygen therapy were the most common risk factors associated with CAM. The mean duration between the onset of symptoms of COVID-19 and onset of CAM was 25.2 ± 15.3 days. Rhino-orbito-cerebral mucormycosis (ROCM) was present in 24.5% of the cases, with a survival rate of 58.3%. Histopathology showed tissue invasion by branching broad-based, pale, fungal hyphae consistent with mucor in 49 (87.5%) cases. Inflammation was acute neutrophilic type in 61.2% of the patients with a survival rate of 63.33%. The survival rate with chronic non-granulomatous and chronic granulomatous was 100% and 81.8%, respectively. Conclusion: Keeping a high index of suspicion for mucormycosis in COVID-19 by all medical fraternities of the health community in the existence of risk factors is the need of the hour for prompt diagnosis. Biopsy of antral necrotic tissue should be performed immediately after suspicion for histopathological study to confirm the diagnosis of mucormycosis and predict prognosis depending on the type of inflammation incited, fungal morphology, load, and necrosis.Item BARDET BIEDL SYNDROME IN PREGNANCY A CASE REPORT(Era's Lucknow Medical College & Hospital, Lucknow, 2021-06) Kumari, S; Rizvi, S; Singh, A; Ahmad, A; Kashiv, S.Bardet Biedl Syndrome (BBS) is a rare idiopathic autosomal recessive disorder which affects multiple organs and organ systems. In India less than 15 cases have been reported so far. We present case of 41 year old third gravida who presented at 17 weeks of gestation. She had a suspicious history of delayed milestones in the previous child and ultrasound findings of echogenic kidneys, bilateral talipes, and polydactyly. Index child evaluation showed type-6 variant of BBS. Amniocentesis in the present pregnancy showed the same mutation in the fetus. The couple decided on termination of t pregnancy.Item Behaviour of expression of nif HDK and fix ABCX promoters of Rhizobium meliloti in R. leguminosarum.(1988-10-01) Singh, A; Agarwala, R; Kashyap, L R; Kumar, SItem The bias against India in western literature on history of medicine: with special emphasis on public health.(2000-01-13) Singh, AThe article describes a systematic bias against India in Western literature on history of medicine. While many authors have ignored the contributions of India in development of medicine altogether, the others have relegated India's role much behind other civilizations. Unnecessary and deliberate controversies on dating and origin of Ayurveda, primacy of Greek vs. Hindu Medicine and the origin of the practice of variolation have been elaborated by Western authors. Some medical historians, like Siegrist, have tried to give India its due place in the history of medicine. Suitable references of Indian authors have also been quoted to give a comparative and balanced picture. The need for settling this controversy has been emphasized.Item Binary conjugational transfer system of vectors pRK290 and pLAFRI is proficient both ways between Escherichia coli and Rhizobium.(1990-10-01) Singh, R K; Singh, A; Misra, A K; Khanuja, S P; Narula, R; Kumar, SWide host range vector plasmids pRK290 and pLAFRI carrying genomic fragments of Rhizobium are transferable both ways between R. meliloti and R. leguminosarum cells on the one hand and to E. coli cells on the other, in triparental matings involving E. coli cells carrying pRK2013, the helper for Tra functions to the vector plasmids. The vector plasmids pRK290 and pLAFRI can be employed for recovering clones harbored by R. leguminosarum and R. meliloti by transfer to Rhizobium cells by direct matings of the library with them.Item Biochemical properties of Clostridium perfringens type A strains isolated from various sources.(1984-05-01) Ram, S; Kalra, M S; Singh, A; Prasad, R