Browsing by Author "Nair, P M"
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Item Activation of protease activity in rat peritoneal macrophages in protein deficiency: characterization of cathepsin D.(1996-07-01) Smitha, S; Machaiah, J P; Nair, P MRat peritoneal macrophages contained high proteolytic activity that was significantly enhanced under the stress induced by protein deficiency. The aspartyl protease cathepsin D which has been known to be the most active protease in endocytic processes was extracted from the macrophages recovered from control (20% protein fed) and protein deficient (4% protein fed) rats and was affinity purified and characterized further. The cathepsin D from the control sample exhibited better recovery, purification and higher specific activity compared to that from the deficient groups. Apparently the pH optima and heat stability of the enzyme from both the groups were similar. The SDS PAGE profile clearly indicated the presence of greater amounts of active forms of cathepsin D in the deficient samples in vivo itself which reflected in a reduction in Km value of the enzyme. Subtle differences observed in the activity of these macrophage proteases in the protein deficient rats may be partly responsible for the enhanced degradation of macrophage membrane proteins reported earlier.Item Aggregation of banana pyrophosphate fructose 6-phosphate 1-phosphotransferase by glycerol.(1996-12-01) Surendranathan, K K; Kanulkar, S U; Nair, P MAddition of glycerol during purification of banana (Musaceae, Musa cavendishii) pyrophosphate fructose 6-phosphate 1-phosphotransferase [(PFP), EC 2.7.1.90] initiated molecular aggregation of the enzyme. The aggregation process was dependent on the glycerol concentration. The native enzyme (66 kDa molecular mass) showed enhanced activity at 3% (V/V) or less of glycerol concentration. Glycerol concentration between 4 and 5% (V/V) affected a gradual and sequential aggregation of native form of the enzyme. These aggregated forms had molecular masses of 135, 200 and 270 kDa. The 135 and 200 kDa forms were stable for about 72 hrs and prolonged storage over 2 weeks resulted in the formation of the 270 kDa form. Concentration over 5% could reduce the time required for aggregation. Fru2.6 bis P activated the enzyme over ten fold, but did not help in the aggregation process. Studies on the role of glycerol on PFP specific activity suggested a difference in the activation process compared to that by Fru2.6bis P. Replacement of Hepes buffer by Tris increased the Fru2.6 bis P requirement for maximum activation by around 10 fold. Removal of glycerol from the buffer media resulted in almost complete inactivation of the enzyme.Item A biochemical preparation of trans-[14C]cinnamic acid.(1981-10-01) Pendharkar, M B; Nair, P MItem Breath holding spells in a neonate.(1995-10-01) Nair, P MItem Change in blood group phenotype.(1996-04-01) Balachandran, V; Nair, P M; Prasad, KItem Characterization of aerobic desaturation of palmitic acid using resting cells of Alcaligenes faecalis.(1979-08-01) Kshirsagar, S S; Nair, P MItem Collodion babies.(1983-11-01) Nair, P M; Sabarinathan, KItem Congenital ichthyosiform erythroderma and harlequin ichthyosis.(1983-11-01) Nair, P M; Sabarinath, K; Chellam, V GItem Congenital malformations of the cardiovascular system.(1988-09-01) Suguna Bai, N S; Vijayalekshmi, M S; Nair, P MItem Demonstration of gamma irradiation induced synthesis of mRNA in potato buds.(1981-08-01) Ussuf, K K; Nair, P MItem Development of an enzyme catalysing the amidation of proteins in the nuclear fraction from gamma irradiated potato buds.(1978-02-01) Satyanarayan, V; Nair, P MItem Effect of gamma irradiation on chemical and biological properties of lipopolysaccharide from Salmonella typhimurium.(1998-06-10) Naidu, M D; Chander, R; Nair, P MLipopolysaccharide (LPS) from S. typhimurium on exposure to gamma-radiation resulted in decrease in toxicity and was less mitogenic, Silver stained profiles of irradiated LPS on polyacrylamide gels revealed complete loss of its heteropolysaccharides which was confirmed further by analysing lipid A and LPS from Salmonella minnesota Re mutants on SDS-PAGE. Glucosamine and 2-keto 3-deoxy-octonate(Kdo) contents were significantly decreased on treatment. Lipid A obtained by removal of heteropolysaccharides from LPS was less toxic on exposure to gamma radiations.Item Ellis--Van Creveld syndrome.(1983-03-01) Suguna Bai, N S; Joseph, T P; Nair, P MItem Enumeration of macrophage carbohydrates in protein-deficient rats: relation to cell surface properties.(1994-06-01) Machaiah, J P; Smitha, S; Adhikari, H R; Nair, P MIn order to study the effect of protein malnutrition on macrophage glycoproteins the carbohydrate composition of peritoneal macrophages from protein-deficient rats has been studied by paper chromatography and HPLC. The results show that the carbohydrate content of resident cells recovered from protein-deficient group was significantly greater than control and decreased on prolonged incubation. In the protein-deficient samples there was a significant decrease in the content of galactose, fucose and galactosamine known to be binding to specific ligands and increase in glucose and mannosamine. In both control and deficient groups, thioglycollate (TG) elicitation resulted in higher total sialic acid content. Prolonged incubation (18 hr) caused an elevation of sialic acid levels in the resident cells, whereas a drastic reduction was observed in the TG elicited cells. In the protein-fed (20%) group, the cell surface sialic acid which contributes to the negative charge of the cells, reduced significantly on culturing the TG cells but not the resident cells. In the protein-deficient group, this effect was seen in the resident cells also; in the TG cells the cell surface sialic acid was significantly low at the isolation stage suggesting that these cells had become comparatively more positively charged in vivo itself. This observed reduction could be correlated to the enhanced sialidase levels in these cells. These protein deficiency related changes in the carbohydrate composition of macrophages could lead to modification of their receptor activity and charge related functions.Item Epidermolysis bullosa--pyloric atresia syndrome.(1989-03-01) Nair, P M; Chellam, V G; Sabarinathan, KItem An etiological study of congenital malformation in the newborn.(1982-12-01) Suguna Bai, N S; Mascarene, M; Syamalan, K; Nair, P MItem Extravasation injuries prevention, the best policy.(1994-03-01) Nair, P MItem Fatty acid composition of some members of family Achromobacteraceae.(1975-06-01) Ghanekar, A S; Nair, P M
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