Browsing by Author "Kumari, P"
Now showing 1 - 9 of 9
Results Per Page
Sort Options
Item Analogue-resistant mutants of Azotobacter chroococcum derepressed for nitrogenase activity and early ammonia excretion having potential as inoculants for cereal crops.(2000-04-24) Lakshminarayana, K; Shukla, B; Sindhu, S S; Kumari, P; Narula, N; Sheoran, R KSpontaneous mutants resistant to methionine sulfoximine (Msx), methyl alanine (Mal) and methyl ammonium chloride (Mac) were derived from A. chroococcum strain A103. Msx and Mal-resistant mutants expressed 1.73 to 10.98% of the fully derepressed nitrogenase activity when grown in Burk's medium containing ammonium acetate. Mac-resistant mutants did not express nitrogenase activity in ammonium acetate supplemented medium. The mutants excreted ammonia even after 2 days of growth and some mutants excreted more ammonia as compared to the parent. Selected mutants were inoculated on wheat (Triticum aestivum) and barley (Hordeum vulgare) under field conditions. Majority of the derepressed mutants increased grain yield of wheat and barley varying from 1.2 to 33.3%. However, host-dependent effects on grain yield were observed with different mutants. Two mutants, Mal 27 and Mac 19 showed significant increase in grain yields of both the crops. The results suggest that metabolic analogue-resistant mutants of Azotobacter have potential for use as a biofertilizer for cereal crops.Item Association of MDR-TB isolates with clinical characteristics of patients from Northern region of India.(2014-07) Gupta, A; Nagaraja, M R; Kumari, P; Singh, G; Raman, R; Singh, S K; Anupurb, SPurpose: We sought to determine the characteristics and relative frequency of transmission of MDR‑TB in North India and their association with the clinical and epidemiological characteristics of TB‑patients. Materials and Methods: To achieve the objectives PCR‑SSCP, MAS‑PCR and direct DNA sequencing were used against 101 Mycobacterium tuberculosis isolates. Results: Multidrug‑resistant‑TB isolates were found to be significantly higher (P = 0.000) in previously treated patients in comparison to newly diagnosed patients. Further, significant differences (P = 0.003) were observed between different age groups (Mean ± SD, 28.6 ± 11.77) of the TB patients and multidrug resistance. Most frequent mutations were observed at codons 531 and 315 of rpoB and katG genes, respectively, in MDR‑TB isolates. Conclusion: Routine surveillance of resistance to anti‑TB drugs will improve timely recognition of MDR‑TB cases and help prevent further transmission in Northern India.Item Curcuma longa as feed additive in broiler birds and its patho-physiological effects.(2007-03-22) Kumari, P; Gupta, M K; Ranjan, R; Singh, K K; Yadava, RBroiler birds (Vencob chicken of 3 days old) when given feed mixed with powdered rhizome of Curcuma longa (CL; @ 1 g/kg) for 42 days of age, showed significant decrease in serum uric acid and albumin as compared to control, whereas significant increase was recorded in the level of serum total protein and globulin. Level of serum glucose, alkaline phosphatase, aspartate amino transferase and calcium showed no significant variation between the two groups. Micronutrient assay revealed significantly higher level of manganese, zinc, iron and copper in treated group as compared to control group. HA/HI test revealed better humoral response against RD vaccine in CL administered birds. Haematological study showed significantly higher haemoglobin and absolute neutrophil count in treated group. Addition of CL as feed additive also resulted in better growth rate, feed consumption and F:C efficiency in the treated birds. Thus, it could be concluded that powdered CL might be a useful feed additive, since it enhanced the F:C efficiency and had nephroprotective properties.Item Cytogenetic and viral studies in nasopharyngeal carcinomas in patients of Indian origin.(1995-03-01) Kumari, P; Augustus, M; Naik, U; Ravi, V; Shenoy, A M; Rao, C R; Kumar, R VFour cases of undifferentiated nasopharyngeal carcinomas (NPC) (grade III-IV) in patients of Indian origin were investigated for specific chromosome markers and evidence of Epstein-Barr virus (EBV) positivity. Abnormalities involving chromosome #3, like del (3) (p24-pter) and 3q+(q27-qter) were found in these patients, similar to earlier reports in patients of Chinese and Kenyan origin2,4,13 who however were EBV positive, unlike the patients in this study who were EBV negative. Implications of the cytogenetic and serological data in Indian patients with NPC, available for the first time, may throw some light on the etiology of the disease in this ethnic group where nasopharyngeal carcinoma is also endemic.Item Effect of diet and body mass index on the serum lipid profile in healthy premenopausal, perimenopausal, and postmenopausal tribal women of India(International Journal of Medical Science and Public Health, 2019-02) Kumari, P; Bano, M; Sahay, GJBackground: The serum lipid profile in premenopausal and perimenopausal/postmenopausal women gets deranged not only due to hormonal changes in different patterns of menstrual cycle but also due to body mass index (BMI) and dietary influence. Objective: The objective of this study was to determine the effect of BMI and dietary influence in serum lipid profile in among groups of premenopausal and perimenopausal/postmenopausal women. Materials and Methods: A hospital-based study was carried out on total (n = 161) healthy women in different phases of menstrual cycle. Results: Dietary factor has not any influence on the lipid profile in these women and BMI has got an effect to a lesser extent on lipid profile in postmenopausal group. Conclusions: Dietary habits and BMI status do not influence the serum lipid levels as most of the women belonged to low socioeconomic status, subsiding on fiber-rich diet and being more physically active.Item Eye trapped an arachnid: Horrific but true!(All India Ophthalmological Society, 2019-11) Yadav, S; Gupta, N; Kumari, P; Tandon, RItem Prevalence of anaemia in pregnant & lactating women in India.(2006-08-04) Agarwal, K N; Agarwal, D K; Sharma, A; Sharma, K; Prasad, K; Kalita, M C; Khetarpaul, N; Kapoor, A C; Vijayalekshmi, L; Govilla, A K; Panda, S M; Kumari, PBACKGROUND & OBJECTIVES: The prevalence of anaemia during pregnancy and lactation was significantly lower in the National Family Health Survey 1998-1999 (NFHS-2), using the hemocue method for haemoglobin estimation compared to earlier surveys. The present study selected seven States and used the same districts and villages studied in the NFHS-2, to see if the reported reduction in prevalence of anaemia was due to health and nutrition inputs and/or due to a different method for haemoglobin estimation. METHODS: A total of 1,751 women (1,148 pregnant and 603 lactating- exclusively breastfeeding up to 3 months of age), from seven States- Himachal Pradesh and Haryana in north; Assam and Orissa in east; Kerala and Tamil Nadu in south and Madhya Pradesh in central India, were selected. Haemoglobin was estimated by the cyanmethaemoglobin method, so that comparison was possible with earlier studies. Data on socio-demographic characteristics, pregnancy, nutritional status and dietary intakes were collected. RESULTS: Prevalence as well as severity of anaemia was significantly higher in the present study as compared to the NFHS-2 study data. The difference could be due to haemocue method, which gives higher haemoglobin values. The contributing factors found on multiple regression analysis for anaemia in pregnancy and lactation were: literacy, occupation and standard living index of the study women; their awareness about anaemia, its prevention by regular consumption of ironfolate tablets and increase in food intake. Maternal height, age of marriage, parity and foetal loss also contributed to haemoglobin level. There were interstate differences; lower fertility, higher literacy and better diet was observed in Himachal Pradesh as compared to Haryana. The literacy and nutritional status of women in Tamil Nadu was lower than Kerala. The remaining 3 states had poor fertility, lower social living index and nutritional status with >90 per cent women being anaemic in pregnancy and lactation. Low prevalence of severe anaemia in Orissa as compared to Assam was due to availability and consumption of iron folate tablets. The antenatal services in the first trimester and checkup by a doctor, along with availability and consumption of iron folate tablets over 3 months in all the States influenced haemoglobin levels. INTERPRETATION & CONCLUSION: Despite the measures taken to control anaemia in pregnancy and lactation in the last two decades, the severity of nutritional anaemia continues to remain a public health issue of great magnitude, suggesting that these measures have been largely ineffective. The present findings also showed interstate differences particularly in fertility, women education, nutrition status and occupation; availability of antenatal services and iron folate tablets as possible factors responsible for differences in prevalence of anaemia.Item Seroconversion following measles immunisation.(1979-04-01) Mittal, S K; Gulati, A; Kumari, P; Bala, V; Gupta, SItem Spontaneous regression of angiolymphoid hyperplasia with eosinophilia of lacrimal gland in an HIV-positive patient(All India Ophthalmological Society, 2019-08) Kumari, P; Kasturi, N; Nagarajan, G; Senthamizh, T; Ramesh, BK; Bheemanathi, HS