Browsing by Author "Das, S R"
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Item Action of cholesterol on virulence related biochemical functions of Entamoeba histolytica.(1988-11-01) Katiyar, S K; Ghoshal, S; Gupta, A K; Das, S R; Pandey, V C; Sagar, PItem Alloxan diabetes in Swiss mice: activity of Na(+)-K(+)-ATPase and succinic dehydrogenase.(1995-07-01) Mishra, G; Routray, R; Das, S R; Behera, H NThe activities of two enzymes viz: Na(+)-K(+)-ATPase and succinic dehydrogenase (SDH) in brain and liver of alloxan diabetic Swiss albino mice are reported. Alloxan diabetes caused significant decrease in the activity of Na(+)-K(+)-ATPase reflecting reduced glucose transport across the cell membrane. On the contrary, the observed enhanced activity of the enzyme SDH is attributed to increased supply of TCA cycle substrates from accelerated oxidation of fatty acids.Item Atypical lymphoid hyperplasia (lymphoid tumour of indeterminate nature) of orbit--a case report.(1989-10-01) Paul, A; Deb, A K; Chakravarty, S; Das, S RAtypical lymphoid hyperplasia, a rare variety of orbital tumour occurring in a 60 year old male patient is presented with review of literature with special relation to its diagnostic and prognostic difficulties.Item Caesarean Myomectomy is No Longer a Dreaded Job: a Case Control Study in Multiple Specialized Centre.(2013-01) Zeba, D; Das, S R; Fatima, N; Islam, N; Uddin, J; Roy, R KRemoval of uterine myoma during caesarean section (CS) is not commonly done due to fear of severe haemorrhage which may lead to hysterectomy. Contradicting the previous belief many studies have been showing that myomectomy during caesarean section is a safe procedure without significant increase of risk. The objective of this study is to analyze the safety and clinical outcome of caesarean myomectomy. This is a prospective study carried out in different hospitals of Faridpur district, Bangladesh from 01.01.10 up to 31.12.12. Total 16 (study group) patients had undergone caesarean myomectomy. Operation time, peroperative blood loss, post operative complications and length of hospital staying was compared with that of 32 women (control group) with caesarean section alone. The result shows that peroperative blood loss was average 350±100 ml in study group which is 50 ml more than control group. Operation time was 20 minutes more and length of hospital staying was 1 day more than control group. There was no post partum haemorrhage, no hysterectomy was done at the time of CS and there was no maternal or perinatal mortality. So, caesarean myomectomy is safe and convenient to patient and cost effective without increasing any extra risk.Item Cholesterol induced changes in concanavalin A agglutinability of Entamoeba histolytica.(1981-12-01) Prasad, A K; Das, S R; Sagar, PItem Chronic myelocytic leukaemia in a patient with sickle cell anaemia.(1986-11-01) Sarma, P S; Chawda, B K; Das, S RItem Differential lectin agglutinability of virulent and avirulent Entamoeba histolytica strains.(1985-01-01) Prasad, A K; Das, S R; Sagar, PItem The distribution of some enzyme group systems among Bengalis.(1970-07-01) Das, S R; Mukherjee, B N; Das, S K; Blake, N M; Kirk, R LItem Enzyme linked immunosorbent assay for serodiagnosis of amoebiasis in children.(1985-02-01) Jyotsna,; Misra, P K; Saxena, P N; Malik, G K; Das, S RItem Granulocytopenia, jaundice and hepatic coma with disseminated tuberculosis.(1986-08-01) Sarma, P S; Das, S R; Chawda, B K; Rizvi, S A; Chaturvedi, U; Bhagoliwal, S KItem Haemolysis: an in vitro model to investigate tissue necrotic activity of Entamoeba histolytica.(1982-10-01) Prasad, A K; Das, S R; Sagar, PItem High prevalence of amoebiasis in Women Protective Home of Lucknow.(1986-09-01) Anand, P; Das, S RItem In vitro effect of alloxan on Na(+)-K(+)-ATPase and succinate dehydrogenase activities in brain and liver of mice.(1993-04-01) Mishra, G; Das, S R; Routray, R; Behera, H NThe present study reports in vitro inhibition of the activities of enzymes Na(+)-K(+)-ATPase and succinate dehydrogenase by alloxan in brain and liver homogenates of Swiss mice. The Vmax of both the enzymes was reduced in presence of alloxan without any substantial alteration in Km for substrate. Lineweaver Burk's plots showed higher 1/Vmax for alloxan treated samples and convergence of both slopes to intercept-1/Km. The observations pointed to non-competitive type inhibition of the enzymes by alloxan. This may be due to the modification of essential--SH groups present within/adjacent to substrate binding sites by alloxan.Item Isoenzyme pattern of clones of Entamoeba histolytica.(1993-07-01) Srivastava, M; Habibullah, C M; Das, S RThe isoenzyme pattern of clones of E. histolytica isolated from symptomatic and asymptomatic patients of amoebiasis were determined by starch gel electrophoresis. Ten parent cultures (uncloned), 5 from symptomatic and 5 from asymptomatic patients were isolated and established in Robinsons medium as xenic cultures. A total of 100 clones were isolated from parent cultures but only 65 could be established. Starch gel electrophoresis was carried out on the uncloned and cloned cultures. The analysis showed that the 5 uncloned culture from symptomatic patients had 4 isolates with pathogenic zymodeme and 1 isolate with non-pathogenic zymodeme pattern. The presence of beta band and absence of alpha band for phosphoglucomutase (PGM) and a fast moving Hexokinase (HK) band were taken as markers of pathogenicity. The 5 cloned cultures from asymptomatic patients had three isolates with pathogenic and 2 with non-pathogenic pattern. The cloned isolates from symptomatic patients (35) had 20 pathogenic zymodemes and asymptomatic patients (30) had 15 pathogenic zymodemes. Non-pathogenic zymodeme obtained in the present study were I, IV, V, X and XIII and the pathogenic zymodemes obtained were II and XIV. The data of the study indicated that (i) asymptomatic patients also harbour pathogenic zymodemes; and (ii) zymodeme II also coexsist with zymodeme XIV in the Indian population.Item Lipid requirements for axenic cultivation of Entamoeba histolytica.(1980-09) Garg, N K; Das, S RFatty acids, cholesterol and glucose present in axenic medium are utilized by growing Entamoeba histolytica but the amoeba is unable to synthesize cholesterol from [U —14 C — ] glucose although the label is incorporated into the fatty acids and non-saponifiable fractions of the organism. Exogenously-added sonicated dispersions of cholesterol, β-sitosterol, lanosterol, lecithin and lauric, palmitic, linoleic and stearic acids are ingested by the amoebae with subsequent loss in amoeboid movement. After a few hours the movement is regained. Cholesterol, lecithin and the fatty acids stimulate amoebic multiplication but are unable to replace serum in the medium either singly or in combination.Item Lysosomal enzymes in axenically grown Entamoeba histolytica NIH-200 passaged through cholesterol.(1980-01-01) Rai, N K; Das, S R; Garg, N K; Lal, A AItem Nutritional factors in the etiopathogenesis of amoebic liver abscess in golden hamsters.(1984-02-01) Raina, N; Das, S R; Acharya, S K; Tandon, B NItem Preparation of standard amoeba-antigen from axenic Entamoeba histolytica and its use in the serodiagnosis and seroepidemiology of amoebiasis.(1979-09) Das, S R; Kidwai, S A; Gupta, A KA method described for large scale cultivation of Entamoeba histolytica axenically in a modified Diamond's TP-S-1 monophasic medium. Crude amoebaantigen prepared by the ultrasonication of the trophozoites of E. histolytica, was fractionated by sephadex G-200 column into four different fractions. The whole antigen and its different fractions were freeze-dried and upon reconstitution contained approximately 1 · 8 mg N/ml or roughly the equivalent of 10 × 106 amoebae per ml. Both whole antigen and its fractions have been used for the detection of specific antibody in the patients' sera. Rabbits were immunised with the antigen and the immunoglobulins were separated from hyperimmune sera by DEAE-cellulose chromatography and salt fractionations. Sera collected from different categories of amoebiasis patients, amoebic liver abscess, amoebic hepatitis, amoebic dysentery, and asymptomatic amoebiasis, were tested serologically using standard amoeba-antigen for serodiagnosis and epidemiological assay of amoebiasis. Results of the assay showed that standard amoeba-antigen is very useful for diagnosis of invasive amoebiasis.Item Rapid micro-IHA test with FACL-SRBCs in serodiagnosis of amoebiasis.(1986-04-01) Khan, A H; Das, S RItem Relaparotomy in Obstetrics and Gynaecology Department of Faridpur Medical College Hospital - Experience in One Year.(2012-07) Alam, I P; Mahabuba; Das, S RAfter abdominal surgery some patient's needs relaparotomy for complications developed postoperatively. Aim of this study was to determine the risk factors causing re-laparotomy, the indications, management and outcomes of relaparotomy admitting in Obstetrics and Gynaecology department in Faridpur Medical College Hospital, a tertiary referral hospital where all complicated patients are referred for management from surrounding districts hospital. It was a cross sectional study done during the period of January 2011 to December 2011, All the cases among which relaparotomy were done after primary surgery were recorded using a protocol prepared for the study. Data was analyzed. Total 6304 patients were admitted in emergency way in Obstetrics and Gynaecology department of Faridpur Medical College Hospital. Among them 1864 patients undergone emergency operations, here 15 patients needed re-laparotomy. The incidence of re-laparotomy was 0.80%. Indications of relaparotomy included internal hemorrhage, postpartum hemorrhage (PPH), retained foreign body, incorrect diagnosis at first laparotomy, intraabdominal collection of pus and urine, Rectus sheath haematoma and burst abdomen. Often more than one procedure was needed to manage the cases. Re-laparotomy causes much morbidity & mortality with increase in hospital stay and cost. Careful selection of cases for primary operation, expertise of the surgeon, good surgical technique and careful postoperative follow-up can reduce the need for re-laparotomy.