Browsing by Author "Banerjee, K"
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Item Activity of dengue-2 virus and prevalence of Aedes aegypti in the Chirimiri colliery area, Madhya Pradesh, India.(1997-03-01) Mahadev, P V; Prasad, S R; Ilkal, M A; Mavale, M S; Bedekar, S S; Banerjee, KRepeated outbreaks of a suspected viral fever in Chirimiri colliery area, Madhya Pradesh were reported since 1990. The area consists of an agglomeration of sprawling settlements at varying altitudes of 816 to 890 m and it has partial sylvan cover. During a 1992 outbreak 25 patients' sera were tested, of which 13 showed seropositivity to dengue (DEN) by MAC-ELISA test; DEN-2 was isolated from Aedes aegypti collected from two of the eight settlements of the area. The principal vector, Ae. aegypti, was prevalent in all the settlements studied; Breteau indices (BI) varied between 2.5 and 125.0; adult house indices (AHI) between 0 and 60.0%; Ae. albopictus and Ae. vittatus occurred in considerable numbers; Ae. aegypti bred in more containers with nonpotable water than those with potable water; the breeding of this species was noted in a maximum number of cement tanks while mud pots were predominant among the available containers. Paired comparisons between relative prevalence indices showed significant correlation and regression coefficients. Significant association of Ae. aegypti breeding with the households having tap water supply was noted, the relative risk declining with the people's use of well water either exclusively or in combination with other sources of water supply. It was also collected in the nonresidential areas. The role of ecological factors in the maintenance and spread of Ae. aegypti and dengue in these settlements is discussed.Item Acute pancreatitis with ischaemic heart disease--an abnormal electrocardiography.(1988-07-01) Kohli, S C; Garg, M K; Rohtagi, S; Machve, Y V; Banerjee, KItem AIDS--glimpses of African experience.(1993-12-01) Banerjee, KItem Antibodies against Japanese encephalitis virus in insectivorous bats from Karnataka.(1988-06-01) Banerjee, K; Bhat, H R; Geevarghese, G; Jacob, P G; Malunjkar, A SItem Antibodies to Kyasanur forest disease virus in bats in the epizootic-epidemic area and neighbourhood.(1978-09-01) Bhat, H R; Sreenivasan, M A; Goverdhan, M K; Naik, S V; Banerjee, KItem Arthropod tissue culture in India (1967-1988)--a review.(1990-11-01) Pant, U; Banerjee, KItem A bacteriophage M13 based sandwich hybridization assay for the detection of hepatitis B virus in human blood.(1991-04-01) Banerjee, K; Khandekar, PA two step hybridization procedure was developed to detect the presence of hepatitis B virus in blood samples using bacteriophage M13 radiolabelled DNA as probe. During the first step of hybridization, single-stranded bacteriophage M13 tg 130 DNA, with 3.2 kb HBV DNA cloned into it, was hybridized to target HBV DNA immobilized on nitrocellulose membrane filter. In the second step of hybridization, M13 DNA annealed to HBV target is detected with the help of double stranded form of M13 DNA. The assay offers minimum 4- to 6-fold higher sensitivity in comparison to single-step conventional hybridization assays. Additionally M13 DNA offers itself as universal probe.Item Broom stick dermatitis.(2003-01-24) Banerjee, K; Banerjee, RSoft plastic handle cover of newly produced broom stick causing contact dermatitis in reported.Item Certain characteristics of Japanese encephalitis virus strains by neutralization test.(1986-03-01) Banerjee, KItem Characterization of & induction of immune response to anti-idiotypic antibodies for JE virus.(1991-05-01) Ghanekar, S A; Cecilia, D; Kutubuddin, M; Goverdhan, M K; Dandawate, C N; Banerjee, K; Ghosh, S NAnti-idiotypic antibodies (anti-Ids, Ab2s) were prepared by immunizing rabbits with two murine monoclonal antibodies (Ab1) having specificities for two independent haemagglutinin (HA) epitopes on JE virus [viz., Hs-1, monoclonal antibody (MAb) specific for Japanese encephalitis virus (JEV) and Hx-1, MAb common to flaviviruses]. Anti-Hs-1 (S-Ab2) and Anti-Hx-1 (X-Ab2) reacted specifically with the immunizing Ab1. In addition, they could react with other MAbs whose reactivity was similar to their immunizing homologous Ab1. The paratope inhibition assay indicated that both anti-idiotypes recognized paratope related idiotopes on their respective Ab1 and could therefore be designated as Ab2 beta. Experimental animals (Swiss mice, Balb/c mice and guineapigs) immunized with S-Ab2 or X-Ab2 produced anti-JE virus antibodies (Ab3) which could be detected by enzyme linked immunosorbent assay, immunofluorescence, haemagglutination inhibition and neutralization tests. The anti-idiotypes were also found to stimulate a cellular immune response in vitro as assessed by 3H thymidine incorporation by lymphocytes from JE vaccinated individuals and experimentally immunized Balb/c mice. The findings of the present study suggest that both the anti-Id antibodies are homobodies which may act as surrogate antigens to manipulate the immune response against JEV.Item Circulating immune complexes in healthy, HIV-antibody positive subjects.(1991-03-01) Thakar, M; Rodrigues, J; Randive, S; Kulkarni, S; Banerjee, KThe components of the circulating immune complexes (CICs) were characterised in asymptomatic HIV seropositive individuals. Forty four of 214 individuals (20.56%) showed the presence of CICs. Specific HIV anti-HIV CICs were detected in 33 of them (75%). The isotypic specificity of antibodies found to these CICs was measured. IgG and IgA immunoglobulin classes were detected in CICs.Item Cloning & expression of chikungunya virus genes coding structural proteins in Escherichia coli.(1990-09-01) Ranadive, S N; Banerjee, KDNA complementary to the single stranded RNA genome of Chikungunya (CHIK) virus with poly A tract was cloned into the plasmid pGEM-3Zf(-) and 5Zf(+) by blunt end ligation strategy. Clones containing the cDNA inserts were selected by X-gal, IPTG system. They were tested for the expression of structural protein(s) of CHIK virus by in situ enzyme immunoassay and Western blot. The former assay system showed the presence of expressed viral proteins. Analysis of Western blot shows that three structural proteins, E1, E2 and capsid (C) are expressed in Esch. coli. The molecular weights of envelope proteins E1 and E2 were 44-46 Kd and 42-44 Kd respectively, which are lesser than the actual molecular weights of virional proteins (50-52 Kd). This may be due to the absence of glycosylation of these proteins in Esch. coli. In clone no. 382, a high molecular weight protein (56-58 Kd) was observed, which was probably the unglycosylated form of P62 polyprotein coded by the virus during its multiplication. A small protein of MW 6-8 Kd was also expressed in clone nos. 382 and 504, and this appeared to be the unglycosylated form of E3 protein of CHIK virus.Item Comparative efficacy of three measles vaccines in Indian children.(1998-10-02) Banerjee, K; Shaikh, N; Phadke, M; Bedekar, S S; Rauta, S K; Mehta, J MA Comparative study of three types of measles vaccines was undertaken among 1005 children. Of these 527 were vaccinated with the Serum Institute of India (SII) vaccine, 230 with Schwarz (SC) and 248 were vaccinated with Edmonston-Zegreb (EZ) vaccine (imported from Zegreb). Though the majority of children reacted favourably with all the three vaccines (SII: 98.43%; SC: 93.40%; EZ: 93.0%) with a rise in titre, but the percentage of seroconversion was significantly higher with the SII vaccine (p < 0.01). The Schwarz and Edmonston Zagreb vaccines showed significantly less GM titre as compared with the other age group i.e. 9-12 months (p < 0.05). With Serum Institute of India (SII) vaccine the GM titres were almost similar in the different age groups. The overall GM titre obtained with the SII vaccine was significantly higher than the SC vaccine (p > 0.001) as well as the EZ vaccine (p > 0.001). It is of interest to note that among the infants, 22.5% children had measles antibody in them before vaccination.Item Comparative immunogenicity of different hepatitis B vaccines among certain high risk groups in India.(1992-09-01) Chadha, M S; Arankalle, V A; Banerjee, KThe immunogenicity of three different commercially available, well established Hepatitis B vaccines was determined. Three groups of high risk individuals were administered the vaccine, viz, Albugam (20 micrograms), Engerix-B (20 micrograms) and vaccine produced by Cheil Sugar & Co. (3 micrograms) respectively in a schedule of 3 doses as recommended by the manufacturers. A fourth group was administered a combination of the Albugam (2 doses) and Engerix-B (1 dose) vaccine. The sera collected 2 months after the 3rd dose showed seroconversion rates to be 100 percent and 95.34 percent respectively in the group which received the Albugam and Engerix-B vaccines respectively. The group which received a combination of both, the seropositivity rate was also 100 percent. The Chiel Sugar vaccine gave a seroresponse of only 52.38 percent. Geometric mean titres among the groups which received Albugam vaccine (501.30) were comparable to those receiving the combination of Engerix-B and Albugam (442.28). Those who received all 3 doses of Engerix-B vaccine showed a significantly lower GMT as compared to the above tow groups (GMT--43.14, p < .001, P < .001 respectively). The GMT in the group that received Cheil Sugar Vaccine was 3.81. Seroresponse was found to be inversely proportionate to age.Item Comparative susceptibility of three species of mosquitoes to infection with Japanese encephalitis virus.(1983-11-01) Banerjee, K; Deshmukh, P K; Ilkal, M A; Dhanda, VItem Correlation between acetylator phenotype and neuropathy in diabetes mellitus patients.(1982-07-01) Surana, M K; Mathur, A K; Banerjee, K; Agarwal, T DItem Correlation between the number of persons suffering from Kyasanur forest disease and the intensity of infection in the tick population.(1977-08-01) Banerjee, K; Bhat, H RItem Counselling in HIV/AIDS--an Indian perspective.(1995-07-01) Banerjee, K; Biswas, RAN overview of the general principles of counselling for HIV/AIDS are overviewed. Relevance of 'shared confidentiality' in Indian context is recommended.Item Critical evaluation of Kyasanur Forest disease virus neutralizing antibodies found in bats (a preliminary report).(1976-01-01) Gadkari, D A; Banerjee, K; Bhat, H RItem Cross-reactivity of antigens specified by Japanese encephalitis & West Nile viruses.(1987-04-01) Banerjee, K; Deshmane, S L