Thai Cancer Journal
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Browsing Thai Cancer Journal by Author "Aree Prasitthipayong"
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Item Correlation between Serum AFP Level and HBsAg, Age and Sex in Subjects of the National Cancer Institute, Thailand(Thai Cancer Journal, 2010-04-07) Aree Prasitthipayong; Anant Karalak; Vichuda Triratapichat; Phongphan RomhirunHepatocellular carcinoma (HCC) is one of the most common cancer in Thailand. Serum alpha-fetoprotien (AFP) is widely used for HCC screening and has been suggested as a tumor marker for monitoring and diagnosis of hepatocellular carcinoma (HCC) in high-risk population. In this study, we aimed to demonstrate the correlation between serum AFP level and HBsAg, age and sex in HCC and normal subjects of the National Cancer Institute, Thailand. The laboratory data from computerized records of the laboratory information system (LIS) were collected. Two groups of sample comprised 545 non-HCC subjects as control group (215 with viral hepatitis B infection and 330 without viral hepatitis B infection) and 61 patients with HCC. Elevation of serum AFP level (\> 20 ng/ml) was detected in 0.9% in controls and 62.3% in HCC patients. In addition, high serum AFP levels were found in 2.3% of controls who were infected with hepatitis B virus. Mean age was higher in controls who had high serum AFP levels, and male had higher serum AFP level than female. In HCC patients, no correlation of serum AFP level with HBsAg with age and sex was found. From our findings, 2.3% of the HBsAg carriers had elevation of AFP level, therefore, these carriers should be further investigated for early detection of HCC.Item Detection of GSTT1 Polymorphisms in Cancer Patients by Real-Time PCR(Thai Cancer Journal, 2010-04-07) Danai Tiwawech; Somjin Chindavijak; Adisak Sornprom; Wutthi Sumetchotimaytha; Aree Prasitthipayong; Yaninee Jarratwisarutporn; Takufumi IshidaCancer is a huge public health problem in Thailand. Glutathione S-transferase T1 gene (GSTT1) plays a crucial role in prevention of cancer by encoding GSTT1 enzyme to detoxify the electrophiles form of carcinogens. GSTT1 polymorphism has been reported to be associated with several malignancies and able to be used as a potential genetic risk marker for cancer. However, GSTT1 polymorphism detection using the conventional polymerase chain reaction (C-PCR) assay is not suitable for a mass screening since it is time consuming and not for safe since it uses a carcinogen in the post PCR. To date, real-time PCR (R-PCR) assay has been proposed as a quicker and safer method to solve these problems. This study aims to establish the R-PCR assay by using SYBR green I fluorescence and melting curve analysis for GSTT1 polymorphism detection in cancer patients by confirming the results of this assay with the results of the C-PCR assay. Two-hundred DNA samples, extracted from peripheral blood leukocyte of Thai patients with cancers of nasopharynx, lung, breast, colon and liver (40 cases in each group) were examined for GSTT1 polymorphism, GSTT1 normal genotype (GSTT1+) and GSTT1 null genotype (GSTT1-) by using the R-PCR assay with SYBR green I and melting curve analysis and the C-PCR assay. The results of GSTT1 polymorphism detection by the R-PCR assay were in concordance with the C-PCR assay (Kappa value, K=1.0). One hundred-forty-one individuals with GSTT1+ in the R-PCR assay showed 2 peaks of melting point at 91.0°C and 88.5°C that correlated with the appearance of 2 DNA bands of GSTT1 [480 base pair (bp)] and β-globulin (268 bp) in the C-PCR assay, respectively. By contrast, fifty-nine individuals with GSTT1- in the R-PCR assay showed a peak of melting point at 88.5°C that associated with the appearance of 1 DNA band of β-globulin (268 bp) in the C-PCR assay. In addition, it has been found that the R-PCR assay was a faster method for GSTT1 polymorphism detection than the C-PCR assay. The present study suggests that the R-PCR with SYBR Green I and melting curve analysis may be a useful screening tool for more convenient, rapid, reliable and safer detection of GSTT1 polymorphism in patients with cancer as compared to the C-PCR assay.Item Effect of Hepatitis C Virus Infection on the Risk of Non-Hodgkin’s Lymphoma in Thailand(Thai Cancer Journal, 2010-04-07) Aree Prasitthipayong; Patcharin Homcha-em; Pattarapong Pornsopone; Somnuk Temiyasathit; Chaiyaporn Kunka; Tawin Klinvimol; Rungsinoppadol Thotong; Chatchai Ekpanyaskul; Suleepron SangrajrangAlthough a high prevalence of hepatitis C virus (HCV) infection among non-Hodgkin’s lymphoma (NHL) patients had been reported, subsequent epidemiological studies conducted to examine a casual association between HCV and NHL have provided inconsistent results across studies. The risk of lymphoma associated with the detection of HCV infection was estimated. Two hundred and sixty nine cases with histopathologically confirmed NHL and 334 controls were included during 2006-2007 in 4 cancer centers in Thailand. All participants underwent a personal interview and blood sampling. Unconditional logistic regression were used to estimate the odds ratio (OR) and 95% confidence interval (95% CI) for NHL associated with HCV. HCV infection were detected in 6/269 (2.2%) among cases and in 6/334 (1.8%) among control subjects. HCV infection showed no association with NHL, OR=1.22 (95% CI=0.38-3.94).In conclusion, no direct correlation can be deduced between NHL and HCV infection in our study.